» Animal Biotechnology Division

Major Research Achievements

Prophylactics

DNA vaccines

  1. A RNA replicon based DNA vaccine vector for enhanced heterologous gene expression in mammalian cells was developed and evaluated to express reporter genes (e.g. Green Fluorescent Protein (GFP) and β-galactosidase) and protective immunogenic genes (e.g. rabies glycoprotein, canine parvovirus VP2).
  2. A bicistronic DNA vector encoding HN and F gene of Newcastle disease virus was constructed.
  3. Construction of bicistronic DNA vector encoding 35 kDa, 16.8 kDa (thiol peroxidase) and 10 kDa PE proteins of Mycobacterium avium paratuberculosis individually along with IFNγ and study of their immunogenicity in mouse model.

Subunit Vaccine

  1. Recombinant epsilon toxoid of Clostridium perfringens as vaccine for enterotoxeimia.
  2. Recombinant OMPs of Pasteurella multocida as vaccine candidate against HS.
  3. Development of sub-viral particles of infectious bursal disease virus in yeast expression system as vaccine candidate against infectious bursal disease.

Therapeutics

  1. Replication-defective adenoviruses and lentiviruses encoding siRNA targeting rabies virus (RV) nucleoprotein (N), glycoprotein (G) and polymerase (L) genes have been constructed and evaluated for their anti-rabies effect in vitro in BHK-21 cells and in vivo in mice.

Peptide-nucleic acids (PNAs)

  1. Newcastle disease virus specific PNAs were designed and evaluated for their ability to inhibit virus replication as well as their use in detection of viral RNA.

Viral gene construct

  1. Molecular pathways for apoptosis caused by NDV and CPV-2 elucidated.
  2. Oncolytic potential of NDV was established in vivo in Rous sarcoma virus (RSV) induced tumour.
  3. Oncolytic potential and molecular pathway involved in apoptosis cased by VP3 gene of CAV and NS1 gene of CPV-2 was established in vitro in HeLa cells.

Diagnostics

Recombinant antigen based

  1. Recombinant VP2 antigen based latex agglutination test for sero-diagnosis of Infectious bursal disease of poultry was developed.
  2. Recombinant haemagglutinin antigen developed in prokaryotic system for detection of antibodies against Newcastle disease of poultry.
  3. Recombinant fiber antigen developed in prokaryotic system for detection of antibodies against Hydropericardium syndrome of poultry.
  4. Recombinant nucleoprotein antigen based single serum dilution ELISA was developed in yeast expression system for sero monitoring of Newcastle disease of poultry by screening a total of 1182 sera samples.
  5. Recombinant B cell antigens of Mycobacterium avium paratuberculosis for detection of antibodies in paratuberculosis infected animals using ELISA based assay.
  6. Recombinant multi-antigenic proteins of Mycobacterium avium paratuberculosis as diagnostic reagent.
  7. Recombinant T cell antigen an alternative to johnin (PPD) for testing in M.a.paratuberculosis (Map) sensitized animals.

PCR based

  1. A single tube reverse-transcription (RT) PCR based diagnostic test was developed for detection of Rabies in suspected brain samples.
  2. RT-PCR (one step and two steps both) were developed for detection of IBD,bluetongue, Newcastle disease and classical swine fever viruses from clinical samples.
  3. RT-PCR in combination with RFLP was optimized for characterization of NDV isolates in lentogenic, mesogenic and velogenic strains and its pathotyping directly from clinical samples.
  4. PCR based detection of BHV-1 (IBR) from bovine serum.
  5. A rapid, sensitive and user friendly visual LAMP based assay was developed for detection of IBR virus in bovine semen.
  6. PCR based detection of Mycobacterium avium paratuberculosis in clinical samples.
  7. PCR based amplification and detection of Clostridium perfringens epsilon toxin from clinical samples.
  8. PCR based amplification of Clostridium perfringens DNA for toxinogenic typing.

Synthetic peptide based

  1. PPR specific peptides identified for detection and quantification of PPRV specific antibodies
  2. A lateral flow penside assay for PPR virus detection using high affinity monoclonal antibodies fabricated on colloidal gold particles was developed.
  3. PNA based point-of-care visual test for viral RNA detection, quantification and pathotyping of NDV.

Molecular Phylogeny

  1. The Classical Swine Fever Virus (CSFV) lapinised vaccine strain was characterized by complete genome sequencing (GenBank Accession no. EU857642).

Technology/patent developed

  1. Synthetic peptide antigen for diagnosis of Peste des petits ruminants (PPR) - Dr Satish Kumar, Sameer Shrivastava, R.K.Singh, and M.P.Yadav (Patent filed vide No 2134/Del/2007).
  2. Multiple antigenic peptide (MAP) assay for detection of Peste des petits ruminants (PPR) virus specific antibodies - Dr Satish Kumar, Sameer Shrivastava, R.K.Singh, and M.P.Yadav (Patent filed vide No 2135/Del/2007).
  3. “An artificially synthesized peptide”. Dr Satish Kumar, Dr. M.V.Bais, Dr. R.S.Kataria and Dr. M.P.Yadav. International DCT publication No. WO/2008/041047, US-patent application no. 12/311554
  4. Recombinant antigen based rapid sero-diagnosis of infectious bursal disease (IBD) – Dr. Sohini Dey, Dr. C. Madhan Mohan and Dr. J.M. Kataria (Patent filed vide No. 491/DEL/2008).
  5. Pestivirus replicase based self-replicating RNA-replicon vector for heterologous gene expression in mammalian cells. Dr. Praveen K. Gupta and Dr. Chhabi Lal Patel (Patent filed vide No 3805/Del/2011).
  6. A rapid sensitive and user friendly visual LAMP-based assay for detection of infectious bovine rhinotrachaitis (IBR) virus in bovine semen. Dr. Praveen K. Gupta, Dr. Sachin S. Pawar, Chetan D. Meshram, Dr. B.P. Mishra (Patent filed vide No 627/Del/2012).
  7. Recombinant antigen based sero-diagnosis of Newcastle disease - Dr. C. Madhan Mohan, Dr. Sohini Dey and Dr. J.M. Kataria (Patent filed vide No. 628/DEL/2012).
  8. Generation of Newcastle disease virus strain R2B from cDNAs by reverse genetics technology. Madhan Mohan, C. and Sohini Dey. Patent application No.300/DEL/2015 filed with the Indian Patent Office, New Delhi.

Technology Transfer

CIF IVRI
  • A rapid immunoassay for sero-diagnosis of infectious bursal disease (IBD).
  • Recombinant nucleoprotein based single serum dilution ELISA kit for sero-diagnosis of Newcastle disease.








Awards/Recognition in the last 5 years

S.No. Awards/Recognition Nos.
1. Commonwealth Academic staff fellowship 3
2. Commonwealth student fellowship 1
3. BOYSCAST fellowship 1
4. DBT Overseas fellowship 2
5. Young scientist awards 3
6. ICAR Lal Bahadur Shastri Young scientist Award 1
7. DBT Travel Grant 1
8. ICMR Travel Grant 1
9. The Houghton trust Travel Grant 1
10. Shyma Singh Balamati memorial award 1
11. ISVIB F.M Burnett award 1
12. ISVIB Alfred Linghard Award 1
13. DBT-CREST Award 1
14. Institute Best Teacher Award 1