» Immunology Section

Major Achievements

  • Dephosphorylated lipopolysaccharide (LPS) of Pasteurella multocida was found to be safe in chicken
  • Bacterial flagellin and CpG motif have proved as an ideal adjuvant for birds
  • Mycobacterium paratuberculosis glycolipid was found to be more specific and less cross reactive
  • LPS and genomic DNA containing CpG from Salmonella Gallinarum activated innate immune system of chickens and gave higher protection after immunization with inactivated Newcastle disease virus vaccine (NDV) to birds on challenge
  • Chitosan coated cationic PLG nanoparticles with mean size of around 165 nm have been prepared for the delivery of Brucella L7/L12 DNA vaccine, that could be absorbed on to the surface of the nanoparticles
  • Transformed fibroblasts antigen was found to be much superior antigen for detection of anti-avian leucosis virus antibodies in the serum samples of chicken flocks, as compared to gsAg, as determined in an indirect ELISA
  • Challenge study indicated saponin adjuvanted Pasteurella multocida vaccine gave better protection than CpG adjuvanted vaccine
  • Receptor mediated antigen delivery through Fc receptor technique by conjugating flagellin protein with Fc fragment has shown enhanced immune response in mice
  • PLG nanoparticles encapsulating outer membrane proteins (OMP) of Salmonella Gallinarum induced mucosal immunity (IgA antibody) at local sites after oral immunization in chickens, indicating that this could be used as a suitable mucosal vaccine delivery system
  • Development of variable envelope (gp85) gene based molecular assays, viz., polymerase chain reaction (PCR) and reverse transcription (RT)-PCR for differential diagnosis of avian leukosis virus (ALV) from Marek’s disease virus (MDV), and reticuloendotheliosis virus (REV) infections in chicken
  • The short term culture filtrate proteins of an Indian field isolate of bovine TB (M. bovis ICC 391, virulent strain) was prepared and characterized for its diagnostic and immuno-prophylactic potential
  • Poly-lactide-co-glycolide (PLG) microsphere based vaccines for three important viral diseases (viz. RD, IBD and EDS-76) of poultry were developed by incorporating or microencapsulating the viral vaccine antigens in the PLG microspheres and their efficacy has been studied in one NATP-CGP project.
  • Developed a recombinant penton protein based indirect ELISA for the detection antibodies against Egg-drop-syndrome (EDS76) in chicken
  • Demonstrated the synergistic interaction of LPS (TLR4 agonist) and resiquimod (TLR7 agonist) in chicken (both in vitro and in vivo conditions)
  • Adjuvant potential of resiquimod (R-848) with inactivated NDV vaccine was demonstrated in SPF chicken
  • Resiquimod was proven to have prophylactic potency against very virulent infectious bursa disease virus (vvIBDV) challenge in chicken
  • Truncated recombinant VP2 protein based latex agglutination assay for detection of serum antibodies against canine parvovirus.
  • Truncated recombinant VP2 protein based immunochromatographic assay and Dot-ELISA for detection of Canine parvovirus.
  • Partial sequencing of VP2 gene of canine parvovirus isolates (2012-2016) revealed the of predominance of new CPV-2a isolates in Bareilly region