» Veterinary Public Health Division

Major Achievements

VPH IVRI
VPH IVRI
VPH IVRI

Discovered a new and potent Brucella phage – Izatnagar (Iz) phage Gr 6.


Diagnostic technologies generated:

  • Improvised enrichment broths/media for isolation of Salmonella, Listeria, Aeromonas and Campylobacter jejuni.
  • Compared and fractionated different antigens of Brucella spp. for developing rapid and reliable tests including Plate ELISA for brucellosis.
  • Indirect IgG ELISA for sero-diagnosis of Japanese encephalitis in swine.

Standardised:

  • Native LLO, synthetic LLO and InlC peptide based ELISA for Listeriosis.
  • ELISA for JE in pigs & brucellosis in small ruminants.
  • ELISA employing rOMPC antigen for Salmonella in faecal samples.
  • PCR protocols for Salmonella, Aeromonas, Listeria, Brucella, Campylobacter, Staph. aureus, Arcobacter and diarrhoeagenic Escherichia coli pathotypes.
  • Rapid 16S rRNA RFLP approach for identification of eight enteric bacterial pathogens at genus level.
  • LAMP test for Arcobacter butzleri and Salmonella enterica subsp. enterica serovar Typhimurium.
  • RT-PCR for human and bovine rotavirus & JE virus.
  • RT-LAMP/Real Time RT- PCR targeting NS1 gene for JE virus.
  • Trans-PCR/Com1/ Real Time PCR & Com1 based LAMP PCR for Coxiella burnetii.
  • Immunoassays (FAT/ELISA/slide agglutination test) for Salmonella, Aeromonas & Listeria spp.
  • Modified Agglutination Test (MAT) for sero-diagnosis of animal and human toxoplasmosis.
  • PCR assay standardized for detection of biofilm associated genes (flaAB, peb1A, peb4, luxS and cj0511) of Campylobacter spp.
  • Optimized PCR for detection of various antimicrobial and virulence genes of S. aureus and E. coli

Findings documented

  • Ameliorative effect of Curd and Arjuna feeding on atherosclerosis lesions in lab animals
  • Nucleotide sequences of OMP 31 gene of B. melitensis isolates from India did not differ revealing its conserved nature
  • Isolation of C. burnetii from bloody faeces of a free roaming bird
  • Genotyping & partial sequencing of rotavirus revealed presence of reassortant & uncommon strains
  • Highest occurrence of C. jejuni biotype-1 followed by C. jejuni biotype-2 and C. coli biotype-1
  • Predominance of Enteroaggregative E. coli from diarrhoeal cases of human infants and young animals
  • Campylobacter in biofilms exhibited many fold resistance against antibiotics than planktonic cells
  • Antimicrobial resistance genes such as blaTEM, blaCTXM, blaAmpC and Sul1 in E. coli, and mec A and blaZ in S. aureus has been detected