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Scientist Profile

M.V.Sc. Ph.D.,
Senior Scientist,
Veterinary Public Health Division,

Telephone: 0581-2301326
Mobile No.: 9412672852

Field of Specialization Veterinary Public Health (Food safety and Zoonoses) Total Research Papers 42
No. Patents Granted / Filed 0 Total No. of Book / Manual 7
No. of M.V.Sc. Student Guided 5 No. of PhD Student Guided 0
Present Number of M.V.Sc. Student 1 Present Numberof PhD Student 3

Top 10 Research Papers

  • Rawool, D.B., Malik, S.V.S., Shakuntala, I., Sahare, A.M. and Barbuddhe S.B. (2006). cDetection of multiple virulence associated genes in Listeria monocytogenes isolated from bovine mastitis cases, International Journal of Food Microbiology 113(2): 201-207.
  • D.B. Rawool, Bitsaktsis, C., Y. Li, D. R. Gosselin, Y. Lin, N.V. Kurkure, D.W. Metzger, and E.J. Gosselin (2008). Utilization of Fc Receptors as a Mucosal Vaccine Strategy against an intracellular bacterium, Francisella tularensis, J. Immunology 180: 5548–5557.
  • Bitsaktsis, C., D. B. Rawool, Yili Li., N. V. Kurkure, B. Iglesias, and E. J. Gosselin. (2009). Differential requirements for protection against mucosal challenge with Francisella tularensis in the presence versus absence of Cholera Toxin B and inactivated F. tularensis, J. Immunology 182(8):4899-4909
  • Vergis, J., Negi , M., Poharkar, K., Das, D.P., Malik, S.V.S., Kumar, A., Doijad S.P., Barbuddhe, S.B., Rawool, D.B. (2013). 16S rRNA PCR followed by restriction endonuclease digestion: A rapid approach for genus level identification of important enteric bacterial pathogens. J. Microbiol. Methods, 95: 353–356.
  • Doijad, S.P., Barbuddhe, S.B., Garg, S., Poharkar, K.V., Kalorey, D.R., Kurkure, N.V., Rawool, D.B. and Chakraborty, T. (2015) Biofilm-Forming Abilities of Listeria monocytogenes Serotypes Isolated from Different Sources. PLoS ONE 10: e0137046. doi:10.1371/journal.pone.0137046 (N
  • Negi, M., Vergis, J., Vijay, D., Dhaka, P., Malik, S.V.S., Kumar, A., Poharkar, K.V., Doijad, S.P., Barbuddhe, S.B., Ramteke, P.W. and Rawool, D.B. (2015) Genetic diversity, virulence potential and antimicrobial susceptibility of Listeria monocytogenes recovered from different sources in India. FEMS Pathogen & Disease, 73: doi: 10.1093/femspd/ftv093
  • Nair, A., Rawool, D.B., Doijad, S., Poharkar, K., Mohan, V., Barbuddhe, S.B., Kolhe, R., Kurkure, N.V., Kumar, A., Malik, S.V.S. and Balasaravanan, T. (2015). Biofilm formation and genetic diversity of Salmonella isolates recovered from clinical, food, poultry and environmental sources. Infection, Genetics and Evolution
  • Kumar, M., Dhaka P., Vijay D., Vergis J., Mohan V., Ashok Kumar, Kurkure N.V., Barbuddhe S.B., Malik S.V.S., Rawool D.B.* (2016). Antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus against multi-drug resistant Enteroaggregative Escherichia coli. International Journal of Antimicrobial Agents. 48: 265 – 270
  • Barbuddhe S.B.*, Rawool D.B., Malik S.V.S., et al., (2016). Presence of a widely disseminated Listeria monocytogenes serotype 4b clone in India. Emerging Microbes and Infections 5, e55; doi:10.1038/emi.2016.55
  • Rawool D.B., Doijad S.P., Poharkar K.V., Negi M., Kale S.B., Malik S.V.S., Kurkure N.V., Chakraborty T., Barbuddhe S.B.*(2016). A multiplex PCR for detection of Listeria monocytogenes and its lineages. Journal of Microbiological Methods 130:144–147

Technologies Developed

  • A diagnostic kit (Multiplex PCR kit) for rapid and reliable detection of virulent Listeria monocytogenes in food and clinical samples. The developed kit was submitted to ICAR, which was released in the ICAR General Body Meet held on March 06, 2012 at NASC Complex, New Delhi.
  • Synthetic LLO peptide based indirect ELISA for serodiagnosis of listeriosis in caprine, ovine and bovine.
  • Real Time and LAMP PCR targeting hly gene for detection of Listeria monocytogenes.
  • Real Time and LAMP PCR targeting IS1111/com1 gene for detection of Coxiella burnetii.
  • LAMP PCR for detection of ‘typical’ and ‘atypical’ Enteroaggregative E. coli by in house design primers targeting fimA gene


  • Overseas Postdoctoral Research Fellowship at Dept of Immunology and Microbial Disease, Albany Medical College, Albany, NY, USA.
  • Overseas Postdoctoral Research Fellowship at Institute for Medical Microbiology, Justus-Liebig University, Giessen, Germany
  • Editorial Member for Journal Foodborne and Zoonotic diseases
  • Editorial Member (Special Edition): Advances in Animal and Veterinary Sciences
  • Associate Editor for Frontiers in Microbiology (Host pathogen Interaction)
  • Reviewer Excellence Award (ARCC Journals)
  • Best oral presentations (02) to my students during IAVMI International Conference held at DUVASU, Oct 30 – Nov 1, 2014, Mathura.
  • Best poster presentation (01) to my student during IAVMI International Conference held at DUVASU, Oct 30 – Nov 1, 2014, Mathura.
  • Best poster presentation (02) to my students during XIV NAVS Convocation-cum-Conference on Antimicrobial resistance in Livestock Health and Production jointly organized by NAVS, ICAR-IVRI and ANA, India at ICAR-IVRI, Izatnagar on 4th Nov., 2015.


  • Indian Association of Veterinary Public Health Specialists
  • Indian Society for Veterinary Medicine
  • Karnataka Veterinary Association
  • American Society for Microbiologists
  • Society for Research on Listeria

Ongoing Projects

  • New Promising approaches for synergistic blocking of anthrax toxin at protective antigen interacting domains of the lethal factor and edema factor
  • Maintenance and supply of stock strains, disease diagnosis and investigation of food and water borne diseases
  • New Promising approaches for synergistic blocking of anthrax toxins at protective antigen interacting domains of the lethal factor and edema factor.
  • Translational Centre for Molecular Epidemiology of Listeria monocytogenes

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