» BIOPHYSICS, ELECTRON MICROSCOPY & INSTRUMENTATION SECTION

Salient Achievements

  • Ultrastructural mechanism of typhoid-like Salmonella (3,10:r:-) infection was worked out (Figure 1).
  • A revolutionary process of vesicular exocytosis in prokaryotes was discovered, by which, the invading gram-negative organisms modify the activities of the host/ target eukaryotic cells by translocating biochemical signals via bacterial outer membrane bounded vesicles (OMVs).
  • A new functional organelle, namely, periplasmic organelle, was discovered in the secretion-active state of gram-negative organisms and its molecular model was also proposed. Acquisition of these organelles, also renders the organisms functionally virulent.
  • A nanovescular model for type III secretion system of gram negative organisms was proposed which radically reforms the current views on its working as a syringe-like microinjection system.
  • Salmonella (3,10:r:-) enterotoxins (sonicated cell-free extract) was shown to exit from ileum by opening inter-epithelial cell membrane tight junctions to enter circulation and thus severely damage liver by centri-lobular necrosis (also suggestive of apoptosis of hepatocytes). Circular dichroism studies suggested loss of 3-dimensional structure of membrane proteins during enterotoxin-microsomal membrane interactions.
  • Neem-seed oil was found to be a cost-effective, biodegradable, eco- and user- friendly termite-repellent for use as aerosol-spray, injection into crevices and surface-painting at locations such as the infested crops, ends of termite-tunnels and affected furnishings, respectively.
  • Static magnetic field created obliquely across the long bone-fracture was shown to augment the healing of fractures due to active alignment of collagen fibers, enhanced hydroxyapatite crystal formation and speedier development of blood vascular network.
  • Novel, nano-scale, bioactive, surface plasmon resonance biosensors’ matrix platform for ultra-sensitive detection of biomolecules and organic substances have been developed.