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Pathology Division
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Major Achievements
Major Achievements
Malignant catarrhal fever, Infectious bovine rhinotracheatis, M. agalactiae in goat mastitis, M. bovigenitalium in cattle with granular vulvo- vaginitis, porcine circovirus-2, and porcine Parvovirus reported for first time in India.
Chlamydial abortions in animals and its association with pneumonia, arthritis and encephalitis established.
Japanese encephalitis virus isolated from stillborn piglets with hydrocephalus and hydranencephaly first time and was sequenced.
Pathogenesis of Streptococcus suis was established in laboratory mice model.
Maedi / Visna virus propagated in sheep choroid plexus culture cells.
Molecular prevalence and pathology of various diseases like JD, Swine influenza, CSF, Jaagsiekte sheep retrovirus, BRV, BCoV, BPV, EBH were established by using conventional and recent techniques like AGID, ELISA, PCR, Realtime PCR, ISH, histopathology, IHC, TEM etc.
A modified Periodic Acid Schiff reagent developed which reduced the maturing time from 4 days to 4 hrs.
Cancer biomarkers p53, c-Myc, PCNA, hTERT, cadherins and fibronectin were studied in canine species. AgNORs, PCNA, Ki-67, c-Myc, c-erbB2 and ER-α markers were studied in spontaneous canine and induced rat mammary tumors. Prevalence of triple negative canine mammary tumours were investigated
Mycotoxicoses: Ochratoxin-A and aflatoxin B1 contamination detected in common feeds/ ingredients in various states. Teratogenic effects T-2 and Citrinin in rats and rabbits was determined. Mannan oligosaccharide and Ascorbic acid along with AT-A were found to ameliorate the toxic effects.
Registry of Pathology houses more than 1020 gross pathological specimens and 1163 color transparencies. The repository has system-wise (10) catalogue of all pathological specimens. Digitalization of specimens initiated.
Avian Disease Laboratory
Developed MATSA test for Marek’s disease, COFAL kit for ALSV infection, Dot-ELISA kit for RD vaccinal immunity etc.
Developed thermostable IBD vaccine strains and EDS-76 vaccine strain.
A new histochemical technique for the differentiation of quail heterophils and eosinophils in tissues.
Production, standardization and testing of live cell culture vaccines against MD, IBD, Reo, IB, ILT and Turkey pox, inactivated, oil-based tissue culture and/or embryo origin vaccines against ND, IBD, Reo, EDS-76, IBH and DVH, combined inactivated vaccine against ND, EDS-76 and IBD. Reovirus, vvIBDV and ALV subgroup “A” isolated for the first time in the country.
Multiplex PCR for MDV, ALV-A and REV, a LAMP test for MDV-1 oncogene meq and a novel diagnostic technique slide ELISA for NDV detection developed.
Role of virally modified exosomes in Marek’s disease viral replication in activated T-cells has been elucidated in vitro.
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