Diagnostic technologies generated

• Developed and released of IgG and IgM ELISA kits for sero-diagnosis of Japanese encephalitis in swine and equines
• Compared and fractionated different antigens of Brucella spp. for developing rapid and reliable tests including Plate ELISA for brucellosis.
• Improvised enrichment broths/media for isolation of Salmonella, Listeria,Aeromonas, and Campylobacter jejuni.
• A diagnostic kit (Multiplex PCR kit) for rapid and reliable detection of virulent Listeria monocytogenes in food and clinical samples. The developed kit was released in the ICAR General Body Meet held on March 06, 2012 at NASC Complex, New Delhi.
• Developed nucleic acid based assays for diagnosis of JE in swine and mosquitoes

Diagnostic Methods Standardised

Diagnostic Methods Standardised

• ELISA for JE in pigs in pigs and equines.
• RT-PCR for genotyping of JE virus.
• RT-LAMP/Real Time RT- PCR targeting NS1 gene for JE virus

Rotavirus

• RT-PCR for detection & genotyping of human, porcine & bovine rotaviruses
• Latex agglutination test for detection of human & bovine rotaviruses

Brucellosis

• ELISA for brucellosis in small ruminants
• biotyping and genotyping (MLVA, MLST) of Brucella spp. isolates
• Indirect hemolysis test was standardized for diagnosis of bovine brucellosis

Listeriosis

• Native LLO, synthetic LLO and InlC peptide based ELISA
• Latex agglutination test for serodiagnosis of listeriosis in bovine and caprine, and for detection of Listeria monocytogenes in foods and clinical samples
• Real Time and LAMP PCR targeting hly gene for detection of L. monocytogenes

Toxoplasmosis

• Modified Agglutination Test (MAT) for sero-diagnosis of animal and human toxoplasmosis

Coxiella burnetii

• IS1111/com1 gene and Trans-PCR/Com1/ Real Time PCR & Com1 based LAMP PCR for detection of Coxiella burnetii.

Antimicrobial resistance

• Optimized PCR for detection of various antimicrobial and virulence genes of Staphylococcus including S. aureus &CoNS, Campylobacter spp., K. pneumoniae and E. coli

Foodborne pathogens

• ELISA employing rOMPC antigen for Salmonella in faecal samples.
• PCR protocols for Salmonella, Aeromonas, Listeria, Brucella, Campylobacter, Staph. aureus, Arcobacter and diarrhoeagenic Escherichia coli pathotypes.
• Rapid 16S rRNA RFLP approach for identification of eight enteric bacterial pathogens at genus level.
• LAMP test for Arcobacter butzleriand Salmonellaenterica subsp. enterica serovar Typhimurium.
• Immunoassays (FAT/ELISA/slide agglutination test) for Salmonella, Aeromonas&Listeria spp.
• PCR assay standardized for detection of biofilm associated genes (flaAB, peb1A, peb4, luxS and cj0511) of Campylobacter spp.

Findings documented

• Discovered a new and potent Brucella phage – Izatnagar (Iz) phage Gr 6
• Ameliorative effect of Curd and Arjuna feeding on atherosclerosis lesions in lab animals
• Nucleotide sequences of OMP 31 gene of B. melitensis isolates from India did not differ revealing its conserved nature
• Isolation of C. burnetii from bloody faeces of a free roaming bird
• Genotyping & partial sequencing of rotavirus revealed presence of reassortant & uncommon strains
• Highest occurrence of C. jejuni biotype-1 followed by C. jejuni biotype-2 and C. coli biotype-1
• Predominance of Enteroaggregative E. coli from diarrhoeal cases of human infants and young animals
• Campylobacter in biofilms exhibited many fold resistance against antibiotics than planktonic cells
• Antimicrobial resistance genes such as AmpC, blaTEM, CTX-M-1, blaSHV-1, qnrA, qnrS, qnrB, sul1, dhfr1, tetA and tetBin E. coli, and mec A and blaZ in S. aureus has been detected
• Virulence genes viz.nuc, coa, clfA, spA, FnbAand enterotoxin producing genes such as sea, eta, etb, see, seg genes and biofilm forming ability genes such as icaA, icaB, icaC, icaD, clfA, clfB, fnbA of S. aureus were characterized.
• Sero-surveillance in different parts of country to assess status of JE in swine and equine population of India

Patents filed

• Recombinant NS1 protein based indirect IgG ELISA for sero-surveillance of Japanese Encephalitis, and kit thereof’. Application No. 201611024016. Published on 16.02.2018
• Patent granted for “A chitosan based biopreservative mix for buffalo meat mince at 5±1°C”. Application No. 2144/DEL/2007(Patent number 278630)